Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B.

Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments

This dataset contains operational taxonomic units for soil eukaryotes from the Wolf and Tamar catchments . A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken. The exact location of each sample site was determined using a Garmin GPS12. Soil samples were kept in the cold and removed to the laboratory for analyses.

Publication date: 2014-05-01

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Supporting documentation

Format of the dataset : Comma-separated values (CSV)

Access and use conditions

This resource is made available under the terms of the Open Government Licence

You must cite: Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/4bf6228f-ce3d-449e-9438-c4b5c8291256

 

© Centre for Ecology & Hydrology (Natural Environment Research Council)

Where/When

Study area
Temporal extent
2013-10-01    to    2014-03-20

Provenance & quality

Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Correspondence/contact details

Point of Contact
Centre for Ecology & Hydrology
Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
United Kingdom
enquiries@ceh.ac.uk

Authors

Griffiths, R.
Centre for Ecology & Hydrology
Pywell, R.F.
Centre for Ecology & Hydrology
Peyton, J.
Centre for Ecology & Hydrology
Nuttall, P.
Centre for Ecology & Hydrology
Sarlej, R.
Centre for Ecology & Hydrology
Thomson, B.
Centre for Ecology & Hydrology

Other contacts

Custodian
Environmental Information Data Centre
Publisher
NERC Environmental Information Data Centre

Spatial

Spatial reference system
OSGB 1936 / British National Grid

Tags

Topic categories
Biota , Environment , Inland Waters , Location
INSPIRE Theme
Land use
Keywords
Acreman Section Biodiversity Environmental monitoring facilities,  genetic information,  Hydrography,  microbiology,  Soil,  Soil South West project,  Species distribution,  Tellus