Evans, T.M.; Heard, M.S.; Vanbergen, A.J.; Cavers, S.; Ennos, R (2017). Fitness traits of experimentally selfed and outcrossed Eschscholzia californica plants. NERC Environmental Information Data Centre. https://doi.org/10.5285/551756fd-77ea-479f-8bc9-f0437c6aead6
This dataset contains measures of fitness traits from Eschscholzia californica progeny which were experimentally supplemented with selfed or outcrossed pollen to determine the effects of self-fertilisation on a plant which has a low propensity to self. A glasshouse experiment was conducted using 40 plants. On each plant two flowers were emasculated and the first supplemented with outcrossed pollen and the second with self-pollen. From each supplemented plant, a seed was sowed from the outcrossed fruit and from the selfed fruit. The following fitness traits were recorded; the germination rate, the duration from germination to reproductive maturity (time of first flower), together with the height (cm) and biomass (number of flowers and buds) at reproductive maturity. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants.
We performed a glasshouse experiment using 40 artificially crossed plants. On each plant, we emasculated two flowers and supplemented the first with outcrossed pollen and the second with self-pollen. This involved methodically wiping two dehiscing anthers from a donor plant or the focal plant onto the receptive stigma with dissecting tweezers, before covering it in fine muslin. From each supplemented plant, we sowed a seed from the outcrossed fruit and from the selfed fruit (given that selfed fruits predominantly only produced one seed) into 1L pots. These were then stored under glasshouse conditions before the fitness traits were measured.