This dataset contains pH, turbidity, conductivity and viral concentration information in river and estuarine water, wastewater, sediment and mussel samples collected in the Conwy River and estuary. The aim of data collection was to monitor wastewater contamination in the freshwater-marine continuum. Samples were collected by trained members of staff from Bangor University at four weekly between March 2016 and August 2017. Treated and untreated wastewater samples were collected at four wastewater treatment plants along the Conwy River. Surface water samples were collected at four sites, sediments at three sites and mussels at two sites. The VIRAQUA project was funded by the Natural Environment Research Council (NERC) under the Environmental Microbiology and Human Health (EMHH) Programme (NE/M010996/1)
Publication date: 2018-03-07
The pH, turbidity and conductivity of the water samples was measured directly, whereas viral nucleic acid concentrations of norovirus GI and GII, hepatitis A and E viruses, sapovirus GI, adenovirus and polyomaviruses were determined in concentrated samples using quantitative polymerase chain reaction (qPCR) and reverse transcription PCR (qRT-PCR). As these quantification methods only measure nucleic acid content and give no indication on viral particle integrity and infectivity, a capsid integrity assay for noroviruses was also used. Viral concentrations are expressed in genome copies (gc) in one litre water or one gram sediment/mussel, where one gc refers to the genome of one virus particle. Data were collated into an Excel spreadsheet and exported into a .csv file for ingestion into the EIDC.