Lugli, L.F. et al

Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018

The data consists of potential phosphatase activity released by fine roots in old growth forests in Central Amazon. Fine roots younger than three months were sampled using the ingrowth core technique in a large-scale nutrient fertilisation experiment. Phosphatase activity is given as a mean of the plant community per plot, where five points inside each plot were sampled and separated in two different soil layers (0-10 and 10-30cm).

Samples were collected in February 2018, eight months after the nutrient fertilisation started at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).

The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship)

Publication date: 2020-04-03

Get the data

This dataset is available under the terms of the Open Government Licence

Format of the data: Comma-separated values (CSV)

You must cite: Lugli, L.F.; Quesada, C.A.N.; Andersen, K.M.; Mercado, L.M.; Fuchslueger, L.; Martins, N.P.; Schaap, K. J.; Hartley, I.P. (2020). Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018. NERC Environmental Information Data Centre.


Natural Environment Research Council


Study area
Temporal extent
2018-02-01    to    2018-09-30

Provenance & quality

Root tips, that usually to refer absorptive fine roots directly responsible for nutrient acquisition, were cut from root sampled using ingrowth cores and potential root acid phosphomonoesterase activity was measured within 3 days of root sampling using a fluorimetric microplate assay. Roots were incubated with Methylumbelliferyl-phosphate (MUF) for 30 minutes at ~ 25 ˚C while gently shaking, and after the termination of the reaction fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Root phosphatase activity per plot was expressed in μmol MUF g−1 dry mass h


This dataset is included in the following collections

Amazon Fertilization Experiment (AFEX) in Central Amazon, Brazil (2017 - 2020)

Correspondence/contact details

Laynara Lugli
National Institute of Amazonian Research, Brazil
Prof. Iain Hartely
University of Exeter


Lugli, L.F.
National Institute of Amazonian Research, Brazil
Quesada, C.A.N.
National Institute of Amazonian Research, Brazil
Andersen, K.M.
Nanyang Technological University, Singapore
Mercado, L.M.
University of Exeter
Fuchslueger, L.
University of Vienna, Austria
Martins, N.P.
National Institute of Amazonian Research, Brazil
Schaap, K. J.
National Institute of Amazonian Researches, Brazil
Hartley, I.P.
University of Exeter

Other contacts

Environmental Information Data Centre
NERC Environmental Information Data Centre

Additional metadata

Topic categories
Biota , Environment
Environmental survey Soil
Environmental Monitoring Facilities
Natural Environment Research Council
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Last updated
01 March 2021 12:31