Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018
Cite this dataset as:
Lugli, L.F.; Quesada, C.A.N.; Andersen, K.M.; Mercado, L.M.; Fuchslueger, L.; Martins, N.P.; Schaap, K. J.; Hartley, I.P. (2020). Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018. NERC Environmental Information Data Centre. https://doi.org/10.5285/6529004d-b6e8-429d-ac68-0f5324d7e5d0
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This dataset is available under the terms of the Open Government Licence 
https://doi.org/10.5285/6529004d-b6e8-429d-ac68-0f5324d7e5d0
The data consists of potential phosphatase activity released by fine roots in old growth forests in Central Amazon. Fine roots younger than three months were sampled using the ingrowth core technique in a large-scale nutrient fertilisation experiment. Phosphatase activity is given as a mean of the plant community per plot, where five points inside each plot were sampled and separated in two different soil layers (0-10 and 10-30cm).
Samples were collected in February 2018, eight months after the nutrient fertilisation started at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).
The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship)
Samples were collected in February 2018, eight months after the nutrient fertilisation started at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).
The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship)
Publication date: 2020-04-03
View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)
Format
Comma-separated values (CSV)
Spatial information
Study area
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Temporal information
Temporal extent
2018-02-01 to 2018-09-30
Provenance & quality
Root tips, that usually to refer absorptive fine roots directly responsible for nutrient acquisition, were cut from root sampled using ingrowth cores and potential root acid phosphomonoesterase activity was measured within 3 days of root sampling using a fluorimetric microplate assay. Roots were incubated with Methylumbelliferyl-phosphate (MUF) for 30 minutes at ~ 25 ˚C while gently shaking, and after the termination of the reaction fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Root phosphatase activity per plot was expressed in μmol MUF g−1 dry mass h
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Lugli, L.F.; Quesada, C.A.N.; Andersen, K.M.; Mercado, L.M.; Fuchslueger, L.; Martins, N.P.; Schaap, K. J.; Hartley, I.P. (2020). Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018. NERC Environmental Information Data Centre. https://doi.org/10.5285/6529004d-b6e8-429d-ac68-0f5324d7e5d0
Natural Environment Research Council
Related
This dataset is included in the following collections
Amazon Fertilization Experiment (AFEX) in Central Amazon, Brazil (2017-2020)
Correspondence/contact details
Authors
https://orcid.org/0000-0001-8404-4841
Quesada, C.A.N.
National Institute of Amazonian Research, Brazil
Martins, N.P.
National Institute of Amazonian Research, Brazil
Schaap, K. J.
National Institute of Amazonian Researches, Brazil
Other contacts
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
info@eidc.ac.uk