Functionalisation data of a carbon electrode biosensor for detection of antimicrobial resistance
The dataset consists of results of the electrochemical circle fit of the Nyquist plot generated by NOVA software to measure the Charge Transfer Resistance (RCT). Data demonstrates the curve of real and imaginary resistance against charges transmitted in the media of the carbon electrode biosensor. The change in RCT can detect the presence of the targeted antimicrobial resistance (AMR) DNA. The experiments were conducted by immersing the sensor's electrode in different concentrations of the target AMR DNA to determine the limit of detection.
Publication date: 2022-01-12
- Temporal extent
2021-07-15 to 2021-07-29
Provenance & quality
The fabrication process involves multi LTCC layers, and screen printing of silver reference electrode (RE), carbon layer of working electrode (WE) and counter electrode (CE), and the dielectric layers. The SPCEs were functionalised with a specific (Peptide nucleic acid) PNA probe, by electrografting through in situ generated diazonium cations. Then creating a 4-carboxyphenyl (AP) film on the electrode surface after the modification of SPCEs electrochemical by reduction of the in situ generated 4-carboxyphenyl diazonium salt, using four cyclic voltammetry (CV) scans from +0.4 to -0.6 V at a scan rate of 100mV/s. The AP film then activated via 60 min incubation in 1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide (EDC) and NHS (N-hydroxysuccinimide) in a MES (2-(Nmorpholino) EthaneSulfonic acid), PH 5.0. After, the electrode surface rinsed in MES buffer, and incubated it in amino-modified PNA probe dispersed in droplet of PBS buffer for 60 min at room temperature. Formation of a blocking film by droplet deposition to the electrode of 1% v/v ethanolamine/PBS buffer and incubated for 30 min in room temperature. Finaly, 52 min incubation with complimentary DNA target and electrochemical impedance spectroscopy (EIS) measurements by (Metrohm-Autolab, PGSTAT204/FRA32M), (dRCT 100kHz to 0.3Hz) in a 1mM KCl + 2mM Ferri/Ferrocyanide solution. DNA strand hybridizing to probs lead to an accumulation of negative charge on the electrode surface causing an increase in the charge transfer resistance (RCT).
Carbon screen-printed electrodes on ceramic substrates for label-free molecular detection of antibiotic resistance: Carbon Sensors for Antibiotic Resistance Detection, Journal of interdisciplinary nanomedicine, vol. 1, no. 3, pp. 93-109, 2016, doi: 10.1002/jin2.16. E.A. Obaje, G. Cummins, H. Schulze, S. Mahmood, M.P.Y. Desmulliez, and T.T. Bachmann.
Al Mshelesh, H.
Heriot Watt UniversityHk49@hw.ac.uk