Salmi, P. et al
Photosynthetic pigments in water samples from 20 lochs in Scotland, May-June 2022
Cite this dataset as:
Salmi, P.; Pölönen, I.; Beckmann, D.A. ; Calderini, M.; May, L.; Olszewska, J.; Perozzi, L.; Pääkkönen, S.; Taipale, S.; Hunter, P. (2023). Photosynthetic pigments in water samples from 20 lochs in Scotland, May-June 2022. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/7794cbfa-1d74-4885-b303-8c20f4608728
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This dataset is available under the terms of the Open Government Licence
https://doi.org/10.5285/7794cbfa-1d74-4885-b303-8c20f4608728
This dataset describes the concentrations of photosynthetic pigments (Chlorophylls, carotenoids and phycocyanin) measured by HPLC and spectrophotometry in water samples of 20 lochs in Scotland. Data were collected between May and June 2022.
Publication date: 2023-09-22
View numbers valid from 22 September 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)
Format
Comma-separated values (CSV)
Spatial information
Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Temporal information
Temporal extent
2022-05-01 to 2022-06-30
Provenance & quality
Water samples were collected by grab sampling from the surface water layers. Sampling sites were located along the lake shores, except at Loch Leven, where boats were used to collect samples from the open water.
Chlorophylls and carotenoids were determined with High Performance Liquid Chromatography (HPLC). Phycocyanin was determined by spectrophotometry.
Samples for HPLC and spectrophotometry were collected on glass fibre filters (GF/F Whatman or Fisherbrand MF300) so that the filters were not clogged but were notably coloured.
The HPLC sample filters were folded to enclose the samples and wrapped in aluminium foil before flash-freezing them by submerging them in liquid nitrogen and then storing them at -80 °C. Sample filters for spectrophotometry were stored at -20 °C.
Filters were shipped on dry-ice to the analysis laboratories where they were stored at -80 °C (HPLC) and 20 °C (spectrophotometry) and analysed within 4-6 months of collection. Details of the HPLC protocol and spectrophotometry are given in data_descriptor.docx.
Chlorophylls and carotenoids were determined with High Performance Liquid Chromatography (HPLC). Phycocyanin was determined by spectrophotometry.
Samples for HPLC and spectrophotometry were collected on glass fibre filters (GF/F Whatman or Fisherbrand MF300) so that the filters were not clogged but were notably coloured.
The HPLC sample filters were folded to enclose the samples and wrapped in aluminium foil before flash-freezing them by submerging them in liquid nitrogen and then storing them at -80 °C. Sample filters for spectrophotometry were stored at -20 °C.
Filters were shipped on dry-ice to the analysis laboratories where they were stored at -80 °C (HPLC) and 20 °C (spectrophotometry) and analysed within 4-6 months of collection. Details of the HPLC protocol and spectrophotometry are given in data_descriptor.docx.
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Salmi, P.; Pölönen, I.; Beckmann, D.A. ; Calderini, M.; May, L.; Olszewska, J.; Perozzi, L.; Pääkkönen, S.; Taipale, S.; Hunter, P. (2023). Photosynthetic pigments in water samples from 20 lochs in Scotland, May-June 2022. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/7794cbfa-1d74-4885-b303-8c20f4608728
Correspondence/contact details
Authors
Perozzi, L.
University of Stirling
Other contacts
Rights holders
University of Jyväskylä, UK Centre for Ecology & Hydrology, University of Stirling
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Additional metadata
Keywords
Funding
Natural Environment Research Council Award: NE/R016429/1
Academy of Finland Award: 321780
European Union Award: 776480
Stirling and Clackmannanshire
Scottish Government
Academy of Finland Award: 321780
European Union Award: 776480
Stirling and Clackmannanshire
Scottish Government
Last updated
08 January 2025 13:32