Cite this dataset as
Salmi, P., Pölönen, I., Beckmann, D.A. , Calderini, M., May, L., Olszewska, J., Perozzi, L., Pääkkönen, S., Taipale, S., Hunter, P. (2023). Photosynthetic pigments in water samples from 20 lochs in Scotland, May-June 2022. NERC EDS Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/7794cbfa-1d74-4885-b303-8c20f4608728
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This dataset is available under the terms of the Open Government Licence 
Photosynthetic pigments in water samples from 20 lochs in Scotland, May-June 2022
This dataset describes the concentrations of photosynthetic pigments (Chlorophylls, carotenoids and phycocyanin) measured by HPLC and spectrophotometry in water samples of 20 lochs in Scotland. Data were collected between May and June 2022.
Publication date: 2023-09-22
View record as
Format
Comma-separated values (CSV)
Spatial information
- Study area
-
- Spatial representation type
- Tabular (text)
- Spatial reference system
- OSGB 1936 / British National Grid
Temporal information
- Temporal extent
-
2022-05-01 to 2022-06-30
Provenance & quality
Water samples were collected by grab sampling from the surface water layers. Sampling sites were located along the lake shores, except at Loch Leven, where boats were used to collect samples from the open water.
Chlorophylls and carotenoids were determined with High Performance Liquid Chromatography (HPLC). Phycocyanin was determined by spectrophotometry.
Samples for HPLC and spectrophotometry were collected on glass fibre filters (GF/F Whatman or Fisherbrand MF300) so that the filters were not clogged but were notably coloured.
The HPLC sample filters were folded to enclose the samples and wrapped in aluminium foil before flash-freezing them by submerging them in liquid nitrogen and then storing them at -80 °C. Sample filters for spectrophotometry were stored at -20 °C.
Filters were shipped on dry-ice to the analysis laboratories where they were stored at -80 °C (HPLC) and 20 °C (spectrophotometry) and analysed within 4-6 months of collection. Details of the HPLC protocol and spectrophotometry are given in data_descriptor.docx.
Chlorophylls and carotenoids were determined with High Performance Liquid Chromatography (HPLC). Phycocyanin was determined by spectrophotometry.
Samples for HPLC and spectrophotometry were collected on glass fibre filters (GF/F Whatman or Fisherbrand MF300) so that the filters were not clogged but were notably coloured.
The HPLC sample filters were folded to enclose the samples and wrapped in aluminium foil before flash-freezing them by submerging them in liquid nitrogen and then storing them at -80 °C. Sample filters for spectrophotometry were stored at -20 °C.
Filters were shipped on dry-ice to the analysis laboratories where they were stored at -80 °C (HPLC) and 20 °C (spectrophotometry) and analysed within 4-6 months of collection. Details of the HPLC protocol and spectrophotometry are given in data_descriptor.docx.
Correspondence/contact details
Authors
https://orcid.org/0000-0003-3247-2259
Perozzi, L.
University of Stirling
Other contacts
- Rights holders
-
University of Jyväskylä, UK Centre for Ecology & Hydrology, University of Stirling
- Custodian
-
NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
- Publisher
-
NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
Additional metadata
- Topic categories
- environment
- INSPIRE theme
- Environmental Monitoring Facilities
- Keywords
- carotenoid , chlorophyll , lake , phycocyanin , phytoplankton , spectrophotometry , water quality
- Funding
- Natural Environment Research Council Award: NE/R016429/1
Academy of Finland Award: 321780
European Union's Award: 776480
Stirling and Clackmannanshire
Scottish Government - Last updated
- 01 March 2024 10:57
More information about these numbers
View numbers valid from 22 September 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)