Evans, T.M.; Heard, M.S.; Vanbergen, A.J.; Cavers, S.; Ennos, R.

Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover

This dataset details the paternity of progeny from Eschscholzia californica plants introduced to habitats comprising different floral cover. Data was collected in June 2015 at the Hillesden estate, Buckinghamshire, UK. Plants were genotyped at seven microsatellite markers before being introduced across the study site to form experimental arrays. Experimental arrays comprised of three E.californica plants separated by 1m and arranged in a triangular formation. A total of sixteen arrays were introduced across four 100 hectare replicate blocks, each separated by >500m. At the centre of each block, four experimental arrays were placed at 50m intervals along a 150m transect laid symmetrically across the boundary between an established wildflower patch and bare, fallow ground or grazed grassland (two arrays within the florally rich habitat and two arrays within the florally poor habitat). Upon maturation approximately 10 seeds were collected from each plant and genotyped. Fragment analysis was conducted and alleles were scored on Genemarker. Seeds were then manually scored as outcrossed or selfed and paternity was determined using Cervus. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants.

Publication date: 2017-06-13

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Supporting documentation

Format of the dataset : Comma-separated values (CSV)

Access and use conditions

This resource is made available under the terms of the Open Government Licence

You must cite: Evans, T.M.; Heard, M.S.; Vanbergen, A.J.; Cavers, S.; Ennos, R. (2017). Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover. NERC Environmental Information Data Centre. https://doi.org/10.5285/7b721c07-bc38-4815-8669-4675867663d0

 

© Centre for Ecology & Hydrology (Natural Environment Research Council)

Where/When

Study area
Temporal extent
2015-06-01    to    2015-06-30

Provenance & quality

All open flowers were removed from the E. californica plants prior to their placement in pre-marked locations across the landscape. One bud on each plant was covered in a fine muslin to represent a 'pollinator excluded' flower. Plants remained in the field for 16 days to ensure full anthesis of new flowers and to allow for multiple pollination events. After this period, all fruit were tagged to ensure that only fruit development arising from the period of the field experiment were included in analyses. Plants were then collected and stored under controlled glasshouse conditions until fruit maturation. Ten progeny per plant from each of the field exposed plants were genotyped using 50mg of fresh leaf material and following standardised protocols. The incidence of self-fertilisation in plants from each habitat was calculated manually by individually comparing each successfully amplified progeny against their maternal plant. If, at each of the seven loci, the progeny was a complete match for the maternal genotype, or was homozygote for one of the maternal plants alleles, it was scored as selfed. Alternatively, if any novel alleles were observed in the progeny that were not present in the maternal plant, the progeny was classified as outcrossed. Paternity was determined using Cervus 3.0.7, where each progeny sample was listed detailing alleles at the seven microsatellite loci, specifying the known maternal sample as well as the potential paternal samples.

Correspondence/contact details

Centre for Ecology & Hydrology
Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
United Kingdom
enquiries@ceh.ac.uk

Authors

Evans, T.M.
Centre for Ecology & Hydrology
Heard, M.S.
Centre for Ecology & Hydrology
Vanbergen, A.J.
Centre for Ecology & Hydrology
Cavers, S.
Centre for Ecology & Hydrology
Ennos, R.
University of Edinburgh

Other contacts

Custodian
Environmental Information Data Centre
Publisher
NERC Environmental Information Data Centre

Spatial

Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid

Tags

Topic categories
Environment
INSPIRE Theme
Environmental Monitoring Facilities
Keywords
allele,  Buckinghamshire Californian poppy,  Eschscholzia californica fallow ground,  grazed grassland,  Hillesden parentage,  paternity,  wildflower