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Pena, R.; Tibbett, M.

Physiological measurements and root morphology of poplar trees cultivated with different fungal guilds

https://doi.org/10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b
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This dataset is available under the terms of the Open Government Licence

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This dataset encompasses 1) Detailed measurements of root architecture parameters - numerical values for Total length (cm), Area (cm²), Surface area (cm²), Average diameter (mm); Root volume (cm³), Number of tips, forks, and crossings; and 2) The physiological responses of poplar trees under varying light conditions - measurements of Transpiration rate (mmol m⁻² s⁻¹), Net CO₂ assimilation rate (µmol m⁻² s⁻¹), Intercellular CO₂ concentration (µmol mol⁻¹), Intercellular CO₂ partial pressure (Pa), and Stomatal conductance to water vapour (mol m⁻² s⁻¹). Data were generated in a glasshouse experiment under elevated CO₂ (740 µmol mol⁻¹).
The Root Architecture data was collected when the trees were six months old. Net Photosynthesis Light Curves were recorded at four time points, starting when the poplar trees were two months old and continuing until they were six months old.

The root architecture data were obtained through WinRhizo scanning and software (Regent Instruments Inc., Quebec City, QC, Canada). The physiological measurements were recorded using LI-6800P Portable Photosynthesis System (LI-COR Biosciences, Lincoln, NE, USA). To construct the light curves, measurements were taken starting from a maximum light intensity of 1500 PPFD (µmol m⁻² s⁻¹) and subsequently decreased to 800, 200 and 50 µmol m⁻² s⁻¹ until no light was applied.

In this experiment, micro-propagated poplar clones were cultivated under four different conditions: without fungi (fungal-free plants), with ectomycorrhizal fungi (ectomycorrhizal plants), with saprotrophic fungi (saprotrophic fungal plants), and with both ectomycorrhizal and saprotrophic fungi (fungal plants). The purpose of the experiment was to evaluate plant performance under the influence of different fungal guilds and their interactions.
Publication date: 2025-09-18
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More information

View numbers valid from 18 September 2025 Download numbers valid from 18 September 2025 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Temporal information

Temporal extent
2023-06-01    to    2023-12-01

Provenance & quality

The plant material used was Populus canescens (grey poplar), a hybrid between Populus tremula and Populus alba. The poplar clones were initially aseptically cultivated in Murashige and Skoog (MS) medium and subsequently transferred to a sandy substrate. The trees were harvested at six months old. The entire root system was collected and immediately scanned using the WinRhizo system. The obtained images were automatically processed using the device's internal software, ensuring accuracy and consistency. No additional data processing was required. Physiological measurements were conducted regularly at four time points during the growth period in the sandy substrate, from when the trees were two months old until they reached six months. Data were collected and processed using the LI-6800P system's internal software, ensuring no additional data processing was necessary. Once collected, the data were controlled to ensure integrity and accuracy and then transferred to the data bank.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Pena, R.; Tibbett, M. (2025). Physiological measurements and root morphology of poplar trees cultivated with different fungal guilds. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b

Correspondence/contact details

Rodica Pena
University of Reading
 rodica.pena@reading.ac.uk

Authors

Pena, R.
University of Reading
Tibbett, M.
University of Reading

Other contacts

Rights holder
University of Reading
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
INSPIRE theme
Environmental Monitoring Facilities
Keywords
Biodiversity , ectomycorrhiza , light curves , poplar clone , saprotrophs , Soil , WinRhizo
Funding
Natural Environment Research Council Award: NE/W005816/1