Sparks, A.M. et al

Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014

These data consist of relative telomere length (RTL) measures from quantitative polymerase chain reaction, of Seychelles warbler birds on Cousin Island, Seychelles. The data were collected by the Seychelles Warbler Project in 1995-2014. Data include bird identity, sex, age, birth period, qPCR plate identity, RTL, technician, territory, field period, mum ID, dad ID, mum age at conception, dad age at conception, dominant female ID in the natal territory, dominant male ID in the natal territory

Publication date: 2021-07-26

Get the data

This dataset is available under the terms of the Open Government Licence

Format of the data: Comma-separated values (CSV)

You must cite: Sparks, A.M.; Spurgin, L.G.; van der Velde, M.; Fairfield, E.A.; Komdeur, J.; Burke, T.; Richardson, D.S.; Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

 

Where/When

Study area
Temporal extent
1995-01-01    to    2014-12-31

Provenance & quality

Blood samples (~25 μl) were collected and stored at room temperature in absolute ethanol. DNA was extracted using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions with modification of overnight lysis at 37 °C and a final DNA elution volume of 80 μl. DNA concentration and purity were quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific). The following thresholds were applied before samples were included for further analysis: (1) DNA concentration must be at least 15 ng/μl (based on a mean of three measurements), (2) the 260/280 absorbance ratio has to be between 1.8 and 2.0 for acceptable DNA purity, and (3) the 260/230 absorbance ratio must be higher than 1.8. DNA integrity was further validated by visualisation with ethidium bromide after electrophoresis on a 1.2% agarose gel, and all samples with evidence of DNA degradation were re-extracted or excluded. All DNA extractions that passed the above criteria were diluted to 3.3 ng/μl before telomere measurement. We used quantitative polymerase chain reaction to calculate a relative measure of telomere length that describes the amount of telomeric DNA in a sample relative to that of GAPDH, a constantly expressed reference gene.

Citations

Sparks, A. M., Spurgin, L. G., Velde, M. van der, Fairfield, E. A., Komdeur, J., Burke, T., … Dugdale, H. L. (2021). Telomere heritability and parental age at conception effects in a wild avian population. Molecular Ecology. doi: 10.1111/mec.15804 https://doi.org/10.1111/mec.15804

Correspondence/contact details

Professor Hannah Dugdale
University of Groningen
 h.l.dugdale@rug.nl

Authors

Sparks, A.M.
University of Leeds
Spurgin, L.G.
University of East Anglia
van der Velde, M.
University of Groningen
Fairfield, E.A.
University of East Anglia
Komdeur, J.
University of Groningen
Burke, T.
University of Sheffield
Richardson, D.S.
University of East Anglia
Dugdale, H.L.
University of Groningen

Other contacts

Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Rights Holder
Dr Hannah Dugdale
University of Leeds
 H.Dugdale@leeds.ac.uk

Additional metadata

Topic categories
Biota
Keywords
Biomarkers,  Life history,  Senescence,  Seychelles Warbler,  Telomere
INSPIRE Theme
Environmental Monitoring Facilities
Funding
Natural Environment Research Council Award: NE/P011284/1
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Last updated
18 May 2022 12:28