Dataset
Flame retardants in the livers of the Eurasian otter collected from Scotland between 2013 and 2015 (PBMS)

This resource is made available under the terms of the Open Government Licence

Walker, L.A.; Moeckel, C.; Pereira, M.G.; Potter, E.D.; Chadwick, E.A.; Shore, R.F. (2016)
https://doi.org/10.5285/9371ce24-7a5a-4964-93bf-884b42fd5fc3

Data comprise concentrations of polybrominated diphenyl ethers (PBDEs) determined using Gas Chromatograph – Mass Spectrometry (GC-MS) in the liver tissue of a small sample of Eurasian otters (Lutra lutra) that died in Scotland between 2013 and 2015. The otters analysed included adult and sub-adult males and females although there were insufficient sample numbers to test for differences among demographic groups.
Otter liver tissue collected during post mortem by Cardiff University Otter Project (from otters found deceased) was analysed for the Predatory Bird Monitoring Scheme by the CEH Lancaster Analytical Chemistry Facility.
The Predatory Bird Monitoring Scheme (PBMS; http://pbms.ceh.ac.uk/) is the umbrella project that encompasses the Centre for Ecology & Hydrology’s National Capability contaminant monitoring and surveillance work on avian predators. By monitoring sentinel vertebrate species, the PBMS aims to detect and quantify current and emerging chemical threats to the environment and in particular to vertebrate wildlife.

Publication date: 2016-09-14

Get the data

Format of the dataset: text/csv

Use of this resource is subject to these restrictions

This resource is made available under the terms of the Open Government Licence

You must cite: Walker, L.A.; Moeckel, C.; Pereira, M.G.; Potter, E.D.; Chadwick, E.A.; Shore, R.F. (2016). Flame retardants in the livers of the Eurasian otter collected from Scotland between 2013 and 2015 (PBMS). NERC Environmental Information Data Centre. https://doi.org/10.5285/9371ce24-7a5a-4964-93bf-884b42fd5fc3

 

© NERC CEH and Cardiff University

This dataset is part of the following

Where/When

Study area
Temporal extent
2013-06-20    to    2015-01-25

Online Resources

PBMS website
Website for the Predatory Bird Monitoring Scheme (PBMS)

Cardiff Otter Project website
Website for the Cardiff University Otter Project

Supporting information
Supporting information available to assist in re-use of this dataset

Download the data
Download a copy of this data

Quality

Lineage
Otters found dead were examined by Cardiff University Otter Project to determine sex and age. Age-class (adult, sub-adult or juvenile) was estimated from a combination of morphometric data and indicators of reproductive activity. Nutritional and reproductive status, lesions, growths and concretions were noted.
Tissue samples, including the liver, were taken as part of the post-mortem examination. Between 2013 and 2015, livers were collected from 16 otters found dead in Scotland for analysis of PBDE, tri-BDE through nona-BDEs, residues.
Age was estimated using weight and signs of sexual maturity. Females weighing less than 2.1 kg and males weighing less than 3 kg were recorded as juvenile. Females above this weight but with no sign of reproductive activity (nipples not showing, immature uterus) and males with a baculum less than 60 mm in length were recorded as sub-adult. Females above this weight and with signs of having reproduced at least once and males above this weight and with a baculum length greater than or equal to 60 mm were recorded as adult.
Liver samples were analysed at the centralized analytical laboratories at the Centre for Ecology and Hydrology, Lancaster. Concentrations of 32 BDEs (6 tri -BDEs, 6 tetra – BDEs, 6 penta-BDEs,4 hexa-BDEs, 2 hepta-BDEs, 5 octa-BDEs and 3 nona-BDEs) were quantified.
A sub- sample of each liver (~1 g) was thawed, weighed accurately, ground with sand and dried with anhydrous sodium sulphate. Each sample was spiked with labeled recovery standards (13C PBDEs and 13C BFRs) and Soxhlet extracted in DCM for 16 h. A small portion of the extract was evaporated to zero volume and the lipid content was determined gravimetrically. The remaining extract was cleaned using automated size exclusion chromatography followed by deactivated alumina column.
All BDEs were analysed by GCMS. Each extract was spiked with labelled internal standards and 100μl of sample was injected into a GC-MS with programmable temperature vaporization (PTV) inlet. The PTV injector was kept at 55°C for 0.45 min, and heated to 325°C at a rate of 700°C min-1 and kept at 325°C for 5 min. Then the temperature was reduced to 315°C min-1 at a rate of 10°C min-1. The GC-MS had a 25 m HT8 column (0.22 mm internal diameter and 0.25 μm film thickness, SGE Milton Keynes, UK) and the carrier gas was helium (2.0 ml min-1). The temperature programme was: isothermal at 80°C for 2.4 min, 25°C min-1 to 200°C, 5°C min-1 to 315°C and was held at 315°C for 9.8 min.
Residues were quantified as recovery corrected concentrations using an internal standard correction method and calibration curves based on PBDE standards (Greyhound Ltd, Birkenhead, UK and LGC Ltd, Teddington, UK).. Average recoveries for 13C-PBDE recovery standards for BDE 28, BDE 47, BDE 126, BDE 153 and BDE 197 ranged between 94% and 98%.
Liver concentrations of flame retardants are reported as ng/g wet weight (wet wt). When all summed PBDE concentrations were calculated, individual congener concentrations below the limit of detection (non-detected) were assigned a zero value.

Authors

Centre for Ecology & Hydrology
Centre for Ecology & Hydrology
Centre for Ecology & Hydrology
Centre for Ecology & Hydrology
Cardiff University Otter Project
Centre for Ecology & Hydrology

Other contacts

Point Of Contact
Resource Provider
Custodian
Publisher
Distributor

Spatial

Spatial representation type
textTable
Spatial Reference System
OSGB 1936 / British National Grid

Keywords

Topic Categories
Environment
INSPIRE Theme
Environmental Monitoring Facilities
Other keywords
Pollution
Environmental survey
Other keywords
Eurasian otter
Lutra lutra
flame retardant

Dataset identifiers

https://catalogue.ceh.ac.uk/id/9371ce24-7a5a-4964-93bf-884b42fd5fc3
doi:10.5285/9371ce24-7a5a-4964-93bf-884b42fd5fc3

Metadata

Record identifier
9371ce24-7a5a-4964-93bf-884b42fd5fc3
Record created by
Chaplow, J.S.
Environmental Information Data Centre
United Kingdom
Record last updated
2017-02-13T11:49:26