Johnson, D. et al

Arbuscular mycorrhizal fungi diversity data from a grassland microcosm experiment [NERC Soil Biodiversity Programme]

These arbuscular mycorrhizal (AM) fungi diversity data were collected in 2000 as part of an investigation in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 years. The experimental treatments comprised: bare soil; monocultures of the non mycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The experiment was undertaken in microcosms, set up at the University of Sheffield. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP).
The data were collected during a project funded under the NERC Soil Biodiversity Thematic Programme, established in 1999.

Publication date: 2018-03-21

Get the data

Download the data

Supporting documentation

Format of the Non-geographic dataset : Comma-separated values (CSV)

Access and use conditions

This resource is made available under the terms of the Open Government Licence

You must cite: Johnson, D.; Vandenkoornhuyse, P.J.; Leake, J.R.; Gilbert, L.; Booth, R.E., ; Grime, J.P; Young, J.P.W.; Read, D.J. (2018). Arbuscular mycorrhizal fungi diversity data from a grassland microcosm experiment [NERC Soil Biodiversity Programme]. NERC Environmental Information Data Centre.


Registration is required to access this data

Copyright © 2018 University of York

Copyright © 2018 University of Sheffield

Copyright © 2018 University of St Andrews

This nonGeographicDataset is part of the following


Temporal extent
2000-07-03    to    2000-10-31

Supplemental information

Other useful information regarding this nonGeographicDataset:

Website for the NERC Soil Biodiversity Programme
Usher, M.B., Sier, A.R., Hornung, M. and Millard, P., 2006. Understanding biological diversity in soil: the UK's Soil Biodiversity Research Programme. Applied Soil Ecology, 33(2), pp.101-113.
Johnson, D., Vandenkoornhuyse, P.J., Leake, J.R., Gilbert, L., Booth, R.E., Grime, J.P., Young, J.P.W. and Read, D.J., 2004. Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms. New phytologist, 161(2), pp.503-515.


Data were collected as part of the NERC Soil Biodiversity Thematic Programme, which was established in 1999. Samples were collected by students and staff during the microcosm experiment before being processed according to standard protocols, with the data then being transferred to the programme data manager at the Centre for Ecology & Hydrology.


Johnson, D.
University of Sheffield
Vandenkoornhuyse, P.J.
University of York
Leake, J.R.
University of Sheffield
Gilbert, L.
University of St Andrews
Booth, R.E.,
University of Sheffield
Grime, J.P
University of Sheffield
Young, J.P.W.
University of York
Read, D.J.
University of Sheffield

Other contacts

NERC Environmental Information Data Centre
Point Of Contact
Sier, A.
Centre for Ecology & Hydrology
Lancaster Environment Centre, Library Avenue, Bailrigg
United Kingdom
Environmental Information Data Centre
Environmental Information Data Centre


Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid


Topic categories
Instrument keywords
Taxon keywords
Arbuscular mycorrhizal fungi
AM fungi
Theme keywords
terminal-restriction fragment length polymorphism

Dataset identifiers


Information maintained by
Sier, A.
Centre for Ecology & Hydrology
Last updated