Johnson, D. et al

Arbuscular mycorrhizal fungi diversity data from a grassland microcosm experiment [NERC Soil Biodiversity Programme]

These arbuscular mycorrhizal (AM) fungi diversity data were collected in 2000 as part of an investigation in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for three years. The experimental treatments comprised: bare soil; monocultures of the non mycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The experiment was undertaken in microcosms, set up at the University of Sheffield. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP).

The data were collected during a project funded under the NERC Soil Biodiversity Thematic Programme, established in 1999.

Publication date: 2018-03-21

Get the data

Download the data

Supporting documentation

Format of the dataset : Comma-separated values (CSV)

This dataset is made available under the terms of the Open Government Licence

You must cite: Johnson, D.; Vandenkoornhuyse, P.J.; Leake, J.R.; Gilbert, L.; Booth, R.E., ; Grime, J.P; Young, J.P.W.; Read, D.J. (2018). Arbuscular mycorrhizal fungi diversity data from a grassland microcosm experiment [NERC Soil Biodiversity Programme]. NERC Environmental Information Data Centre.


This dataset is part of the following


Temporal extent
2000-07-03    to    2000-10-31

Supplemental information

Other useful information regarding this dataset:

Website for the NERC Soil Biodiversity Programme
Johnson, D., Vandenkoornhuyse, P.J., Leake, J.R., Gilbert, L., Booth, R.E., Grime, J.P., Young, J.P.W. and Read, D.J., 2004. Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms. New phytologist, 161(2), pp.503-515.
Usher, M.B., Sier, A.R., Hornung, M. and Millard, P., 2006. Understanding biological diversity in soil: the UK's Soil Biodiversity Research Programme. Applied Soil Ecology, 33(2), pp.101-113.

Provenance & quality

Data were collected as part of the NERC Soil Biodiversity Thematic Programme, which was established in 1999. Samples were collected by students and staff during the microcosm experiment before being processed according to standard protocols, with the data then being transferred to the programme data manager at the Centre for Ecology & Hydrology.

Correspondence/contact details

Andy Sier
Centre for Ecology & Hydrology
Lancaster Environment Centre, Library Avenue, Bailrigg
United Kingdom


Johnson, D.
University of Sheffield
Vandenkoornhuyse, P.J.
University of York
Leake, J.R.
University of Sheffield
Gilbert, L.
University of St Andrews
Booth, R.E.,
University of Sheffield
Grime, J.P
University of Sheffield
Young, J.P.W.
University of York
Read, D.J.
University of Sheffield

Other contacts

Environmental Information Data Centre
NERC Environmental Information Data Centre


Topic categories
Biota , Environment
AM fungi,  Arbuscular mycorrhizal fungi,  Microcosm,  terminal-restriction fragment length polymorphism,  T-RFLP