Cite this dataset as
Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345
Import this citation into your reference management software:
BibTeX | Reference Manager (RIS) | Endnote
This dataset is made available under the terms of the Open Government Licence
Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013)
This work was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC) and contributed to the Zambia trypanosomiasis case study. The research was funded by NERC project no NE/J000701/1 with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA).
Format
Comma-separated values (CSV)
Spatial information
- Study area
-
- Spatial representation type
- Tabular (text)
- Spatial reference system
- WGS 84
Temporal information
- Temporal extent
-
2013-05-01 to 2013-11-30
Provenance & quality
Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control.
To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).
Related
This dataset is included in the following collections
Correspondence/contact details
Lusaka
ZAMBIA
Edinburgh
Midlothian
EH25 9RG
UNITED KINGDOM
Authors
Other contacts
- Custodian
-
NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
- Publisher
-
NERC Environmental Information Data Centreinfo@eidc.ac.uk
Additional metadata
- Topic categories
- biota
health - Keywords
- Dynamic Drivers of Disease in Africa Consortium (DDDAC) , Glossina morsitans morsitans , Luangwa Valley , Sodalis glossinidius , Zambia
- Funding
- Natural Environment Research Council Award: NE/J000701/1
- Last updated
- 01 March 2024 11:28
More information about these numbers
Get the data
By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.
This dataset is made available under the terms of the Open Government Licence
CITE AS: Simuunza, M.; Anderson, N.E; Machila, N.; Ndebe, J. ; Macleod, E.T.; Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre. https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345
© The University of Edinburgh
© University of Zambia