This data set describes the prevalence of trypanosomes and Sodalis glossinidius, and host blood meal analysis from tsetse flies (Glossina morsitans morsitans) captured during two intensive surveys in Mambwe District, Eastern Province, Zambia in 2013. The Luangwa Valley in Zambia is an old focus of human African trypanosomiasis (HAT, Trypanosoma brucei rhodesiense) and sporadic outbreaks have continued to occur in the human population. In recent years there has been an influx of people migrating from the densely populated plateau region resulting in a significant change in land-use in the study area, potentially influencing tsetse dynamics and the epidemiology of HAT. This data set was collected to monitor infection rates of trypanosomes and Sodalis glossinidius in Glossina morsitans morsitans tsetse flies in the area so as to assess the risk posed to both human and livestock populations. In addition, feeding patterns of tsetse were investigated through analysis of blood meals.
This work was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC) and contributed to the Zambia trypanosomiasis case study. The research was funded by NERC project no NE/J000701/1 with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA).
Publication date: 2017-03-24
This dataset is part of the following
Tsetse surveys were conducted in May / June 2013 and November 2013. Tsetse flies were sampled using black screen fly rounds. Data were recorded on standard field sampling forms and then manually transferred to Microsoft Excel. Data were then checked for accuracy and incorrect spatial location coordinates removed.
Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control.
To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).