Colonisation rates of E. coli on plastic and banana leaf under simulated environmental conditions

Collection/Generation Methods:
Data were generated through laboratory experiments investigating the persistence, and survival of a clinically relevant strain of E. coli. Bacteria were cultured on materials (Banana Leaf and HDPE) and analysed for persistence over time.
Nature and Units of Recorded Values:
The data encompass bacterial strain identity, material type, timepoints (days) and bacterial concentration (CFU/ml). Units are provided as specified in each data file.
Quality Control:
The data underwent rigorous quality control through replication (biological triplicates), statistical analysis (ANOVA), and controlled experimental conditions (e.g., temperature and material consistency).
Details of Data Structure:
The data are organised into separate CSV files, with each file corresponding to a specific experimental variable (e.g., persistence, water properties). Column headers describe the experimental factors (strain, material, etc.), with units listed where applicable.
Experimental Design/Sampling Regime:
E. coli strains were inoculated onto materials in nutrient-rich and surface water environments. Sampling was taken at defined time points (7, 14, 21, 28 days).
Fieldwork and Laboratory Instrumentation:
Materials (Banana Leaf and HDPE) were prepared and inoculated under controlled laboratory conditions. The experiments utilised standard laboratory equipment such as 96-well plates and incubators.
Calibration Steps and Values:
Instrumentation used for bacterial enumeration, including the plate reader, was calibrated according to standard laboratory protocols.
Analytical Methods:
Statistical methods including ANOVA and Tukey’s post-hoc tests were applied to assess treatment effects and survival outcomes.