Provenance & quality
Experimental communities were composed of toad tadpoles, common frog tadpoles, or a 50/50 mixed species community and assigned one of four exposure regimes, which were broken into 2 phases according to pathogen type.
(i) Control: Phase 1 - 6 x sham exposure to sterile media, sham 2 – 1 x sham exposure
(ii) Bd only: Phase 1 - 6 x 150,000 Bd zoospores, phase 2 – single sham exposure
(iii) Rv only: Phase 1 - 6 x sham exposure, phase 2 – single Rv exposure
(iv) Co-infection: 6 x 150,000 Bd zoospores, phase 2 – single Rv exposure
For phase one, Bd zoospores in media or the same volume of sterile media (control) were introduced into the tanks. Phase 2 control tanks received three Rv-negative corpses. For phase three, Rv-positive larval common frog corpses were introduced into treatment tanks.