Fuller, N.; Smith, J.T.; Ford, A.T.

Effects of low-dose ionising radiation on reproduction and DNA damage in marine and freshwater amphipod crustaceans

https://doi.org/10.5285/b70afb8f-0a2b-40e6-aecc-ce484256bbfb Cite this dataset

This dataset is embargoed and will be made available by 19 July 2020 at the latest

Data comprise results of laboratory experiments assessing the impacts of beta radiation (phosphorus-32) on reproduction, development and DNA damage in a marine and freshwater crustacean species. All crustacean samples were collected either from Lock Lake, Portsmouth (marine crustacean Echinogammarus marinus) or from the River Ems, Emsworth (freshwater crustacean, Gammarus pulex). Laboratory experiments were conducted periodically from summer 2015 to autumn 2016 at the University of Portsmouth. The data are of use in elucidating the mechanisms and effects of low-dose ionising radiation on an important group of model organisms in radioecology.

Publication date: 2018-08-22

This dataset is part of the following

TRansfer - Exposure - Effects (TREE) Integrating the science needed to underpin radioactivity assessments for humans and wildlife

Where/When

Study area
Temporal extent: 2015-06-01 to 2016-09-30

Supplemental information

Other useful information regarding this dataset:

Fuller, N.; Smith, J.T.; Ford, A.; Nagorskaya, L.L.; Gudkov, D.I. (2018). Impacts of chronic radiation exposure on reproduction, development and genetic diversity of the freshwater crustacean Asellus aquaticus at Chernobyl. NERC Environmental Information Data Centre

https://doi.org/10.5285/47f036c4-e319-4825-9cb8-f27977eb20dd

Methods for Comet Assay

This paper documents the methods which were adapted to perform the comet assay on amphipod sperm with this dataset.

Comet Assay Analysis

This paper describes the software package that was used to calculate DNA damage in the associated dataset.

Embryo abnormality analysis

This paper describes the methods used within this dataset to analyse embryo abnormalities within amphipods.

Embryo abnormality analysis

This report describes the methods used within this dataset to analyse embryo abnormalities within amphipods.

Does Chernobyl-derived radiation impact the developmental stability of Asellus aquaticus 30 years on? Fuller, N., Smith, J.T., Nagorskaya, L.L., .Gudkovc, D.T. and Ford, A.T.

http://dx.doi.org/10.1016/j.scitotenv.2016.10.097

The biological effects of ionising radiation on Crustaceans: A review. Fuller, N. Lerebours, A., Smith, J.T. and Ford, A.T.

https://doi.org/10.1016/j.aquatox.2015.07.013

Reproduction in the freshwater crustacean Asellus aquaticus along a gradient of radionuclide contamination at Chernobyl. Fuller, N., Ford, A.T., Nagorskaya, L.L., Gudkov, D.I and Smith, J.T.

https://doi.org/10.1016/j.scitotenv.2018.01.309

Provenance & quality

Sperm quality data was ascertained using a LIVE/DEAD sperm viability kit on sperm suspensions obtained by dissection. DNA damage data was obtained using a modified comet assay method.