Fuller, N.; Smith, J.T.; Ford, A.T.

Effects of low-dose ionising radiation on reproduction and DNA damage in marine and freshwater amphipod crustaceans

https://doi.org/10.5285/b70afb8f-0a2b-40e6-aecc-ce484256bbfb Cite this dataset

Data comprise results of laboratory experiments assessing the impacts of beta radiation (phosphorus-32) on reproduction, development and DNA damage in a marine and freshwater crustacean species. All crustacean samples were collected either from Lock Lake, Portsmouth (marine crustacean Echinogammarus marinus) or from the River Ems, Emsworth (freshwater crustacean, Gammarus pulex). Laboratory experiments were conducted periodically from summer 2015 to autumn 2016 at the University of Portsmouth. The data are of use in elucidating the mechanisms and effects of low-dose ionising radiation on an important group of model organisms in radioecology.

Publication date: 2018-08-22

Where/When

Study area
Temporal extent: 2015-06-01 to 2016-09-30

Provenance & quality

Sperm quality data was ascertained using a LIVE/DEAD sperm viability kit on sperm suspensions obtained by dissection. DNA damage data was obtained using a modified comet assay method.

This dataset is included in the following collections

TRansfer - Exposure - Effects (TREE) Integrating the science needed to underpin radioactivity assessments for humans and wildlife

Supplemental information

Other useful information regarding this dataset:

Comet Assay Analysis

This paper describes the software package that was used to calculate DNA damage in the associated dataset.

Embryo abnormality analysis

This paper describes the methods used within this dataset to analyse embryo abnormalities within amphipods.

This paper documents the methods which were adapted to perform the comet assay on amphipod sperm

Lacaze, E., Devaux, A., Mons, R., Bony, S., Garric, J., Geffard, A., & Geffard, O. (2011). DNA damage in caged Gammarus fossarum amphipods: A tool for freshwater genotoxicity assessment. Environmental Pollution, 159(6), 1682–1691.

Report describing methods used within this dataset to analyse embryo abnormalities within amphipods

Fuller, N., Smith, J.T., Nagorskaya, L.L., Gudkov, D.I., & Ford, A.T. (2017). Does Chernobyl-derived radiation impact the developmental stability of Asellus aquaticus 30 years on? Science of The Total Environment, 576, 242–250

https://doi.org/10.1016/j.scitotenv.2016.10.097

Fuller, N., Lerebours, A., Smith, J.T., & Ford, A.T. (2015). The biological effects of ionising radiation on Crustaceans: A review. Aquatic Toxicology, 167, 55-67.

https://doi.org/10.1016/j.aquatox.2015.07.013

Fuller, N., Ford, A.T., Nagorskaya, L.L., Gudkov, D.I., & Smith, J.T. (2018). Reproduction in the freshwater crustacean Asellus aquaticus along a gradient of radionuclide contamination at Chernobyl. Science of The Total Environment, 628–629, 11-17.

https://doi.org/10.1016/j.scitotenv.2018.01.309