Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019

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https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

Fungal community DNA was sequenced from soils in northern, subarctic Sweden. Soils were sampled from treeline mountain birch forest and stands of tundra willow that were wither ‘girdled’ (connection between leaves and roots cut by disrupting the phloem transport) or ‘control’ (no treatment), 0, 1 and 2 years after the treatment was applied.

Publication date: 2022-08-31

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Format

Comma-separated values (CSV)

Spatial information

Study area

Spatial representation type

Tabular (text)

Spatial reference system

WGS 84 / Pseudo-Mercator

Temporal information

Temporal extent

2017-08-03 to 2019-08-05

Provenance & quality

The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling.
DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:

Parker, T.C.; Chomel, M.; Clemmensen, K.E.; Friggens, N.L.; Hartley, I.P.; Johnson, D.; Krab, E.J.; Lindahl, B.D.; Street, L.E.; Subke, J.-A.; Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

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Supplemental information

Parker, T. C., Clemmensen, K. E., Friggens, N. L., Hartley, I. P., Johnson, D., Lindahl, B. D., Wookey, P. A. (2020). Rhizosphere allocation by canopy-forming species dominates soil CO2 efflux in a subarctic landscape. New Phytologist, 227(6), 1818–1830

https://doi.org/10.1111/nph.16573