Part of UKCEH UKCEH logo
UKCEH website
Hodgson, C. J.

E. coli counts of fresh cattle faeces from the North Wyke Farm Platform

Download/Access
PLEASE NOTE: By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.

This dataset is available under the terms of the Open Government Licence

Download the data
https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours.

Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months.
Publication date: 2019-09-13
35 downloads *
705 views *

More information

View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84

Temporal information

Temporal extent
2016-11-15    to    2018-07-17

Provenance & quality

Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10.
Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content.
The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.

Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Hodgson, C. J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123

© Natural Environment Research Council

Correspondence/contact details

Dr. Chris Hodgson
Rothamsted Research
 chris.hodgson@rothamsted.ac.uk

Author

Hodgson, C. J.
Rothamsted Research

Other contacts

Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
environment
farming
INSPIRE theme
Environmental Monitoring Facilities
Keywords
Agriculture , E. coli
Funding
Natural Environment Research Council Award: NE/N019792/1
Last updated
08 February 2024 17:37