Hodgson, C. J.

E. coli counts of fresh cattle faeces from the North Wyke Farm Platform

The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours.

Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months.

Publication date: 2019-09-13

Get the data

This dataset will be available under the terms of the Open Government Licence

Format of the dataset : Comma-separated values (CSV)

You must cite: Hodgson, C. J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123

 

© Natural Environment Research Council

Where/When

Study area
Temporal extent
2016-11-15    to    2018-07-17

Provenance & quality

Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10.
Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content.
The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.

Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.

Correspondence/contact details

Dr. Chris Hodgson
Rothamsted Research
 chris.hodgson@rothamsted.ac.uk

Author

Hodgson, C. J.
Rothamsted Research

Other contacts

Custodian
Environmental Information Data Centre
 eidc@ceh.ac.uk
Publisher
NERC Environmental Information Data Centre
 eidc@ceh.ac.uk

Additional metadata

Topic categories
Biota , Farming , Environment
Keywords
Agriculture Cattle,  E. coli,  Faeces,  North Wyke Farm Platform
INSPIRE Theme
Environmental Monitoring Facilities
Funding
Natural Environment Research Council
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Last updated
2019-09-13