Vihermaa, L.E.; Waldron, S.; Newton, J.; Donets, V.

Carbon and nutrient data for streams in the Peruvian Amazon

Data from two small streams, two rivers and rainfall fractions in the Western Amazonian basin at Tambopata National Reserve in Madre de Dios region, Peru. Data presented are nutrients (calcium, magnesium, potassium, sodium, total soluble phosphorus and silica) and fluvial carbon - dissolved inorganic carbon (DIC) and its isotopic composition δ13C-DIC, dissolved organic carbon (DOC) and particulate organic carbon (POC). Samples were collected during the period from February 2011 to May 2012 targeting both wet and dry seasons.

Samples for DIC samples were collected using pre-acidified evacuated Exetainers. Established standard methods were used to take samples for DOC and nutrients. Established standard methods were used to analyse samples for DIC, DOC and nutrients These methods are outlined in the lineage. The samples were taken to understand the hydrological controls on the carbon concentrations and fluxes during different flow conditions.

The data collection was carried out as part of the Natural Environment Research Council funded Amazonica project.

Publication date: 2016-04-25

Get the data

This dataset is made available under the terms of the Open Government Licence

Format of the dataset : Comma-separated values (CSV)

You must cite: Vihermaa, L.E.; Waldron, S.; Newton, J.; Donets, V. (2016). Carbon and nutrient data for streams in the Peruvian Amazon. NERC Environmental Information Data Centre.


This dataset is part of the following


Study area
Temporal extent
2011-02-11    to    2012-05-22

Supplemental information

Other useful information regarding this dataset:

Waldron, S., E. M. Scott, L. E. Vihermaa, and J. Newton (2014), Quantifying precision and accuracy of measurements of dissolved inorganic carbon stable isotopic composition using continuous-flow isotope-ratio mass spectrometry, Rapid. Commun. Mass Sp., 28(10), 1117-1126.

Provenance & quality

During three field campaigns (Feb.-Apr. 2011, Sept.-Dec. 2011 and Mar.-May 2012) samples for measurement of Dissolved Inorganic Carbon (DIC), Delta 13 Carbon - Dissolved Inorganic Carbon , Dissolved Inorganic Carbon (DOC) and Particulate Organic Carbon (POC) concentrations were collected from the streams and rivers in the study area. On a selected set of samples nutrient analyses were carried out. Different flow conditions were targeted in sampling to understand the hydrological controls on the carbon concentrations and fluxes. DIC samples were collected in pre-acidified (150 micro litre of concentrated phosphoric acid) evacuated 12 millilitre (ml) exetainers (Labco Ltd). Using a 10 ml syringe (rinsed with sample water 3 times) sample was injected into the exetainers and the needle withdrawn under water to prevent air ingress. On each sampling occasion triplicate exetainers were filled. DIC samples were analysed according to the headspace method on Thermo-Fisher-Scientific Gas Bench/Delta V Plus for DIC concentration and isotopic composition Delta 13 Carbon -DIC. Initially two replicate exetainers were analysed (in randomised order) and the third sample used as a backup to check if a good agreement was not achieved between the first two. This technique is further described in Waldron et al. 2014.

DOC samples were collected in polyethylene bottles and filtered through pre-furnaced (8 hours at 450 degrees Celsius ) 0.7 micro metre glassfibre filter paper (Whatman GF/F) on the day of sampling and stored refrigerated in Nalgene bottles. Prior to the analysis aliquots of the samples were acidified to pH 3.9 and degassed to remove any DIC and analysed for DOC concentration by combustion (Thermalox TOC 2020, Analytical Sciences). The filter papers were air-dried and stored in air tight containers with indicating silica gel as a drying agent. POC concentration was measured by loss on ignition. In the laboratory the filter papers were first oven dried (3 hours at 105 degrees Celsius) to establish the dry weight which was measured on a five figure balance. Then the filter papers were furnace (16 hours at 375 degrees Celsius) and re-weighed. The weight loss was converted to mg C assuming percentage Organic Carbon is equal to 50 percent.

Aliquots of the filtered water sample were analysed for calcium (Ca) and magnesium (Mg) by Atomic Absorption Spectrometry (Perkin Elmer Analyst 400). Potassium (K) and sodium (Na) concentrations were determined by flame photometry (Sherwood Scientific M410). Colorimetric method was used to measure total dissolved phosphorus (totP) and silicon (Si). Phosphorus analysis is based on the formation of a phosphomolybdate complex which is then reduced using ascorbic acid to give blue colour that can be measured at 880 or 700 Nano metres (nm). Silica was converted to a coloured compound using the Heteropoly Blue Molybdosilicate method (American Public Health Association, 1999) and measured at 650 nm.

Data was entered into Excel spreadsheets and exported as comma separated files for ingestion into the Environmental Information Data Centre (EIDC).

Correspondence/contact details

Dr. Leena Vihermaa
University of Glasgow
School of Geographical and Earth Sciences
G12 8QQ
United Kingdom


Vihermaa, L.E.
University of Glasgow
Waldron, S.
University of Glasgow
Newton, J.
Scottish Universities Environmental Research Centre
Donets, V.
University of Glasgow

Other contacts

Environmental Information Data Centre
NERC Environmental Information Data Centre


Spatial representation type
Tabular (text)
Spatial reference system


Topic categories
Inland Waters
Amazon,  Amazonia,  Atomic Absorption Spectrometry (Perkin Elmer Analyst 400),  DIC,  Dissolved inorganic carbon,  dissolved organic carbon,  DOC,  nutrients,  particulate organic carbon,  Peru POC,  Thermalox TOC 2020,  Thermo-Fisher-Scientific Gas Bench/Delta V Plus