Ormsby, M.J.; White, H.L.; Metcalf, R.; Oliver, D.M.; Quilliam, R.S.
        
        Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions
         https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
        
       
            Cite this dataset as: 
            
           
          Ormsby, M.J.; White, H.L.; Metcalf, R.; Oliver, D.M.; Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
             
             
            
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 This dataset is available under the terms of the Open Government Licence  
 
          This dataset contains information relating to the survival and persistence of clinically relevant strains of E. coli on plastic and cotton, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model. 
 
This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1].
         This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1].
           Publication date: 2025-04-09
          
         View numbers valid from 09 April 2025 Download numbers valid from 09 April 2025 (information prior to this was not collected)
           
          Format
Comma-separated values (CSV)
Temporal information
          Temporal extent
         
         2023-01-01    to    2023-06-28
          
         Provenance & quality
         Biofilm formation, persistence and pathogenicity of E. coli were investigated through controlled laboratory experiments. Biofilms were generated on various materials in nutrient-rich and surface water environments, quantified using crystal violet staining, and analysed in triplicate. Persistence was assessed by recovering E. coli from biofilms over 28 days, followed by serial dilution, and selective plating. 
 
Pathogenicity was evaluated using Galleria mellonella larvae, with standardised inoculation protocols and survival monitoring for 72 hours. Data underwent thorough validation at each stage, including replication, and selective media confirmation, ensuring high reliability and accuracy before submission.
       Pathogenicity was evaluated using Galleria mellonella larvae, with standardised inoculation protocols and survival monitoring for 72 hours. Data underwent thorough validation at each stage, including replication, and selective media confirmation, ensuring high reliability and accuracy before submission.
Licensing and constraints
 This dataset is available under the terms of the Open Government Licence  
 
         Cite this dataset as: 
         
       Ormsby, M.J.; White, H.L.; Metcalf, R.; Oliver, D.M.; Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
          
          
         
        Related
Correspondence/contact details
Authors
          White, H.L.
         
         
          University of Stirling
         
        
          Metcalf, R.
         
         
          University of Stirling
         
        Other contacts
          Rights holder
         
         
           University of Stirling
          
         
          Custodian
         
         
            NERC EDS Environmental Information Data Centre
           
  info@eidc.ac.uk
          
          Publisher
         
         
            NERC EDS Environmental Information Data Centre
           
  info@eidc.ac.uk
          Additional metadata
          Keywords
         
         
        
          Funding
         
          Natural Environment Research Council  Award: NE/V005847/1   
Natural Environment Research Council Award: NE/S005196/1
        Natural Environment Research Council Award: NE/S005196/1
 
      
 https://orcid.org/0000-0002-3991-2336
 https://orcid.org/0000-0002-3991-2336