Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021
https://doi.org/10.5285/481c424e-f277-4eb7-9ee0-cbdb5532ef79
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This dataset is available under the terms of the Open Government Licence
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The dataset includes markers of oxidative state in banded mongooses at Queen Elizabeth National Park, Uganda, in the period 2017-2021.
Data are: red blood cell concentrations of glutathione; blood plasma concentrations of malondialdehyde; blood plasma concentrations of protein carbonyls; red blood cell activity of total superoxide dismutase; and whole milk concentrations of malondialdehyde.
Data are: red blood cell concentrations of glutathione; blood plasma concentrations of malondialdehyde; blood plasma concentrations of protein carbonyls; red blood cell activity of total superoxide dismutase; and whole milk concentrations of malondialdehyde.
Publication date: 2022-03-08
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Format
Comma-separated values (CSV)
Spatial information
Study area
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Spatial resolution
15 metres
Temporal information
Temporal extent
2017-01-01 to 2021-12-31
Provenance & quality
Individuals were blood sampled from the jugular vein under general anaesthesia. Samples were collected into EDTA-coated Vacutainers before plasma and erythrocytes were separated by centrifugation then snap frozen in liquid nitrogen within 10 minutes of collection. Samples were shipped to the UK within 3 months under cryogenic conditions, and stored at -80C until analysis.
Analyses were performed blindly with respect to sample identity, and all steps were conducted on ice. All chemicals were HPLC grade, and chemical solutions were prepared using Milli-Q water. Assays were conducted within one year of collection.
Malondialdehyde was determined by HPLC with fluorescence detection (Agilent 1000). Protein carbonyls, glutathione and superoxide dismutase were determined using colorimetric assay kits (Cayman Chemical Company) and a Molecular Devices plate reader (Spectramax M2).
Analyses were performed blindly with respect to sample identity, and all steps were conducted on ice. All chemicals were HPLC grade, and chemical solutions were prepared using Milli-Q water. Assays were conducted within one year of collection.
Malondialdehyde was determined by HPLC with fluorescence detection (Agilent 1000). Protein carbonyls, glutathione and superoxide dismutase were determined using colorimetric assay kits (Cayman Chemical Company) and a Molecular Devices plate reader (Spectramax M2).
Licensing and constraints
This dataset is available under the terms of the Open Government Licence 
Cite this dataset as:
Blount, J.D.; Cant, M.A.; Meniri, M.; Thompson, F.J.; Marshall, H.H. (2022). Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/481c424e-f277-4eb7-9ee0-cbdb5532ef79
Related
Correspondence/contact details
Other contacts
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Rights holder
University of Exeter
j.d.blount@exeter.ac.uk