Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments

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This dataset is made available under the terms of the Open Government Licence

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https://doi.org/10.5285/4bf6228f-ce3d-449e-9438-c4b5c8291256

This dataset contains operational taxonomic units for soil eukaryotes from the Wolf and Tamar catchments . A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken. The exact location of each sample site was determined using a Garmin GPS12. Soil samples were kept in the cold and removed to the laboratory for analyses.

Publication date: 2014-05-01

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More information

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Format

Comma-separated values (CSV)

Spatial information

Study area

Spatial representation type

Tabular (text)

Spatial reference system

OSGB 1936 / British National Grid

Temporal information

Temporal extent

2013-10-01 to 2014-03-20

Provenance & quality

Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Licensing and constraints

This dataset is made available under the terms of the Open Government Licence

Cite this dataset as:

Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/4bf6228f-ce3d-449e-9438-c4b5c8291256

BibTeX RIS

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

UK Centre for Ecology & Hydrology

Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM

enquiries@ceh.ac.uk