Population dynamics of evolving bacterial populations in liquid media, 2020
Cite this dataset as:
Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4
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By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.
This dataset is available under the terms of the Open Government Licence 
https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4
This data was collected from co-evolving bacterial populations containing Pseudomonas fluorescens strain SBW25 and a plasmid, pQBR57. The composition of the community was tracked using flow cytometry to distinguish 1) an unlabelled wild type strain 2) a dTomato compensated host (SBW25 KO PFLU4242), and 3) a wild type host bearing a Green fluorescent protein (GFP) labelled compensated plasmid (pQBR57 KO 0059).
Publication date: 2022-11-23
View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)
Format
text
Spatial information
Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Temporal information
Temporal extent
2020-01-01 to 2020-12-31
Provenance & quality
Flow cytometry data was filtered to remove background noise (using thresholds in forward scatter (FSC-H), side scatter (SSC-H) and blue signal (PB450-H) channels), then all events were classified by fluorescence (flow_data.txt) to provide a community composition for each sample recorded as population fractions (fraction_data.txt).
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4
Related
Correspondence/contact details
Author
https://orcid.org/0000-0002-8095-8256 Other contacts
Rights holder
University of Manchester
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Additional metadata
Keywords
co-evolution , plasmid compensation , pQBR57 , SBW25
Funding
Natural Environment Research Council Award: NE/R008825/2
Last updated
21 March 2025 09:19