Hill, M.; Jenkinson, L.A.; Dean, A.P.; Redfern, J.; Shaw, K.J.

Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)

Name: Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)
Published: 2024-06-12

https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491 Cite this dataset

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This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided.

The work was supported by the Natural Environment Research Council (Grant NE/X011607/1).

Publication date: 2024-06-12

Formats

Comma-separated values (CSV)
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Spatial information

Study area
Spatial representation type: Tabular (text)
Spatial reference system: OSGB 1936 / British National Grid

Temporal information

Temporal extent: 2022-06-20 to 2022-10-12

Provenance & quality

Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls.

For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP.

The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.