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Woodford, L.; White, H.; Fellows, R.; Ormsby, M.; Quilliam, R.

Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves

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This dataset is available under the terms of the Open Government Licence

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https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
This dataset contains experimental survival rate and transfer potential measurements of two known human pathogens, bacteria Salmonella Typhimurium and Vibrio cholerae, on plastic mulch and ready-to-eat crop plants. Colony forming units (CFUs) were used to measure persistence time of each pathogen on the mulch, and quantify the transfer of each pathogen from the plastic mulch to the surface of spinach and basil leaves. Samples were measured on days 1, 2, 3, 4, 7, 10 and 14, over a total period of 14 days. Destructive sampling methods were used to collect samples from the plastic and leaf surface, with negative control leaves also sampled at each time point.

This dataset was created as part of a study investigating the risk of pathogen growth in agricultural environments using plastic mulch, and is supported by the National Environmental Research Council (NE/V005847/1).
Publication date: 2025-05-02
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More information

View numbers valid from 02 May 2025 Download numbers valid from 02 May 2025 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Temporal information

Temporal extent
2023-10-01    to    2023-11-01
Temporal resolution

Provenance & quality

Persistence experiments, examining the survival of S. Typhimurium and V. cholerae were set up by placing spinach and basil leaves in petri dishes and adding plastic mulch with a biofilm containing each pathogen to the leaf surface. Over time samples were destructively sampled by removing the plastic, washing the leaf surface and cutting a circle from the leaf. These three sections (plastic, wash and cut leaf) were then resuspended and plated on to selevctive media. Any surviving bacteria were quantified using CFUs. Negative control leaves with no plastics or bacteria were analysed at each time point.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Woodford, L.; White, H.; Fellows, R.; Ormsby, M.; Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135

Supplemental information

Woodford, L., Fellows, R., White, H. L., Ormsby, M. J., & Quilliam, R. S. (2024). Salmonella Typhimurium and Vibrio cholerae can be transferred from plastic mulch to basil and spinach salad leaves. In Heliyon (Vol. 10, Issue 10, p. e31343). Elsevier BV.

Correspondence/contact details

Woodford, L.
University of Stirling
 luke.woodford@stir.ac.uk

Authors

Woodford, L.
University of Stirling
White, H.
University of Stirling
Fellows, R.
University of Stirling
Ormsby, M.
University of Stirling
Quilliam, R.
University of Stirling

Other contacts

Rights holder
University of Stirling
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
environment
INSPIRE theme
Human Health and Safety
Keywords
Agriculture , Environmental risk , human pathogens , phyllosphere , plastisphere , Pollution , ready-to-eat-crops
Funding
Natural Environment Research Council Award: NE/V005847/1
Last updated
07 May 2025 08:24