Robroek, B.J.M.; Shepherd, H.E.R.; Sahar, N.E.; Dumont, M.G.

Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018

https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad Cite this dataset
This dataset contains information on the Bacterial Amplicon Sequence Variant (ASV) abundance from peat soil sampled following a wildfire on the Stalybridge estate (UK) in June 2020. Samples were taken in 10 established plots at three time periods following the fire: approximately 3 months, 10 months and 12 months post-fire. This was taken at two depths: 0 – 5cm from the surface (top) and 5 – 10 cm from the surface (bottom). The 10 plots were divided into two groups of five, one that was observed to have suffered a light burn (termed shallow burn) and one that received a more severe burn (termed deep burn). Five additional plots were sampled at the same time as the 3-month samples from a neighbouring unburned site (termed control plots). Samples where no ASVs were observed were removed from the dataset.
Publication date: 2022-01-18

Format

Comma-separated values (CSV)

Where/When

Study area
Temporal extent
2018-10-01    to    2018-10-31

Provenance & quality

DNA was extracted from the peat soil using the QIAGEN DNeasy powersoil kit, following standard manual specifications. The extracted DNA was then sequenced through the use of high-throughput sequencing, using 515F/806R primers that target the V4 region of the 16S rRNA. Results from the sequencing were then cleaned and prepared using the DADA2 pipeline to produce the ASV table.

Correspondence/contact details

Dr. Bjorn Robroek
University of Southampton
 bjorn.robroek@ru.nl

Authors

Robroek, B.J.M.
Radboud University
ORCID iD icon https://orcid.org/0000-0002-6714-0652
Shepherd, H.E.R.
University of Southampton
ORCID iD icon https://orcid.org/0000-0002-7077-3581
Sahar, N.E.
University of Southampton
Dumont, M.G.
University of Southampton
ORCID iD icon https://orcid.org/0000-0002-7347-8668

Other contacts

Rights holder
Radboud University
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
Biota , Environment
Keywords
16S rRNA,  Bacterial Amplicon Sequence Variant,  Blanket bog,  DNA,  Moorland,  Peatland
INSPIRE Theme
Environmental Monitoring Facilities
Funding
Natural Environment Research Council Award: NE/S011943/1
Spatial representation type
Tabular (text)
Spatial reference system
GB place names
Last updated
30 August 2022 12:19

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By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.

This dataset is available under the terms of the Open Government Licence

Download the data Supporting documentation

CITE AS: Robroek, B.J.M.; Shepherd, H.E.R.; Sahar, N.E.; Dumont, M.G. (2022). Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad