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Robroek, B.J.M.; Shepherd, H.E.R.; Sahar, N.E.; Dumont, M.G.

Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018

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This dataset is available under the terms of the Open Government Licence

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https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad
This dataset contains information on the Bacterial Amplicon Sequence Variant (ASV) abundance from peat soil sampled following a wildfire on the Stalybridge estate (UK) in June 2020. Samples were taken in 10 established plots at three time periods following the fire: approximately 3 months, 10 months and 12 months post-fire. This was taken at two depths: 0 – 5cm from the surface (top) and 5 – 10 cm from the surface (bottom). The 10 plots were divided into two groups of five, one that was observed to have suffered a light burn (termed shallow burn) and one that received a more severe burn (termed deep burn). Five additional plots were sampled at the same time as the 3-month samples from a neighbouring unburned site (termed control plots). Samples where no ASVs were observed were removed from the dataset.
Publication date: 2022-01-18
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More information

View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
GB place names

Temporal information

Temporal extent
2018-10-01    to    2018-10-31

Provenance & quality

DNA was extracted from the peat soil using the QIAGEN DNeasy powersoil kit, following standard manual specifications. The extracted DNA was then sequenced through the use of high-throughput sequencing, using 515F/806R primers that target the V4 region of the 16S rRNA. Results from the sequencing were then cleaned and prepared using the DADA2 pipeline to produce the ASV table.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Robroek, B.J.M.; Shepherd, H.E.R.; Sahar, N.E.; Dumont, M.G. (2022). Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad

Correspondence/contact details

Robroek, B.
University of Southampton
 bjorn.robroek@ru.nl

Authors

Robroek, B.J.M.
Radboud University
Shepherd, H.E.R.
University of Southampton
Sahar, N.E.
University of Southampton
Dumont, M.G.
University of Southampton

Other contacts

Rights holder
Radboud University
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
environment
INSPIRE theme
Environmental Monitoring Facilities
Keywords
16S rRNA , Bacterial Amplicon Sequence Variant , Blanket bog , bog , DNA , Moorland , peat , Peatland
Funding
Natural Environment Research Council Award: NE/S011943/1
Last updated
21 March 2025 13:19