Griffiths, R.; Edwards, F.; Scarlett, P.; Webb, G.; Sarlej, R.; Thomson, B.

Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments

This datset contains operational taxonomic units for epilithon eukaryotes (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Publication date: 2014-05-06

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Supporting documentation

Format of the dataset : Comma-separated values (CSV)

Access and use conditions

This resource is made available under the terms of the Open Government Licence

You must cite: Griffiths, R.; Edwards, F.; Scarlett, P.; Webb, G.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367

 

© Centre for Ecology & Hydrology (Natural Environment Research Council)

Where/When

Study area
Temporal extent
2013-10-01    to    2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Correspondence/contact details

Edwards, F.
Centre for Ecology & Hydrology
Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
United Kingdom
enquiries@ceh.ac.uk

Authors

Griffiths, R.
Centre for Ecology & Hydrology
Edwards, F.
Centre for Ecology & Hydrology
Scarlett, P.
Centre for Ecology & Hydrology
Webb, G.
Centre for Ecology & Hydrology
Sarlej, R.
Centre for Ecology & Hydrology
Thomson, B.
Centre for Ecology & Hydrology

Other contacts

Custodian
Environmental Information Data Centre
enquiries@ceh.ac.uk
Publisher
NERC Environmental Information Data Centre
eidc@ceh.ac.uk

Spatial

Spatial reference system
OSGB 1936 / British National Grid

Tags

Topic categories
Biota , Environment , Inland Waters , Location
INSPIRE Theme
Land use
Keywords
Biodiversity Environmental monitoring facilities,  genetic information,  Hydrography,  microbiology,  Reynard Section Soil,  Soil South West project,  Species distribution,  Tellus