Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B.

Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments

Name: Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments
Published: 2014-05-06
License: https://eidc.ceh.ac.uk/licences/OGL/plain

https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b Cite this dataset

This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken.

Publication date: 2014-05-06

Where/When

Study area
Temporal extent: 2013-10-01 to 2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

Dr. Robert Griffiths

UK Centre for Ecology & Hydrology

Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM

enquiries@ceh.ac.uk