Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B.

Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments

This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken.

Publication date: 2014-05-06

Get the data

This dataset is made available under the terms of the Open Government Licence

Format of the data: Comma-separated values (CSV)

You must cite: Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

 

© UK Centre for Ecology & Hydrology

Where/When

Study area
Temporal extent
2013-10-01    to    2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Related

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

Dr. Robert Griffiths
UK Centre for Ecology & Hydrology
Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM
 enquiries@ceh.ac.uk

Authors

Griffiths, R.
Centre for Ecology & Hydrology
Pywell, R.F.
Centre for Ecology & Hydrology
Peyton, J.
Centre for Ecology & Hydrology
Nuttall, P.
Centre for Ecology & Hydrology
Sarlej, R.
Centre for Ecology & Hydrology
Thomson, B.
Centre for Ecology & Hydrology

Other contacts

Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
 info@eidc.ac.uk
Resource Provider
Centre for Ecology & Hydrology
 enquiries@ceh.ac.uk
Rights Holder
UK Centre for Ecology & Hydrology
 enquiries@ceh.ac.uk

Additional metadata

Topic categories
Biota , Environment , Inland Waters , Location
Keywords
genetic information,  microbiology,  South West project,  Tellus
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Last updated
20 May 2022 10:41