Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments

https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

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This dataset is made available under the terms of the Open Government Licence

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This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken.

Publication date: 2014-05-06

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Format

Comma-separated values (CSV)

Spatial information

Study area

Spatial representation type

Tabular (text)

Spatial reference system

OSGB 1936 / British National Grid

Temporal information

Temporal extent

2013-10-01 to 2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Licensing and constraints

This dataset is made available under the terms of the Open Government Licence

Cite this dataset as:

Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

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