Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
Cite this dataset as:
Connolly, J. A.; Del Carratore, F.; Schmidt, K.; Bisesi, A.; Martinson, J. N. V.; Chua, J.; Kuhs, M.; Boneza, M.; Heinsch, S.; Kinkel, L.; Smanski, M.; Harcombe, W. R.; Breitling, R.; Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
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This dataset is available under the terms of the Open Government Licence 
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https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures.
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
Publication date: 2025-02-12
View numbers valid from 12 February 2025 Download numbers valid from 12 February 2025 (information prior to this was not collected)
Format
.mzML
Provenance & quality
The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Connolly, J. A.; Del Carratore, F.; Schmidt, K.; Bisesi, A.; Martinson, J. N. V.; Chua, J.; Kuhs, M.; Boneza, M.; Heinsch, S.; Kinkel, L.; Smanski, M.; Harcombe, W. R.; Breitling, R.; Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Related
Supplemental information
Correspondence/contact details
Authors
https://orcid.org/0000-0003-3643-5578
Schmidt, K.
University of Manchester
Bisesi, A.
University of Minnesota
Martinson, J. N. V.
University of Minnesota
Chua, J.
University of Manchester
Kuhs, M.
University of Minnesota
Boneza, M.
University of Minnesota
Heinsch, S.
University of Minnesota
Smanski, M.
University of Minnesota
Harcombe, W. R.
University of Minnesota
Other contacts
Rights holder
University of Manchester
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Additional metadata
Keywords
Funding
Natural Environment Research Council Award: NE/T010959/1
National Science Foundation Award: USA, 1935458
National Science Foundation Award: USA, 1935458
Last updated
19 February 2025 15:14