Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
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This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures.
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
Publication date: 2025-02-12
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Format
.mzML
Provenance & quality
The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
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This dataset is available under the terms of the Open Government Licence 
Cite this dataset as:
Connolly, J.A.; Del Carratore, F.; Schmidt, K.; Bisesi, A.; Martinson, J.N.V.; Chua, J.; Kuhs, M.; Boneza, M.; Heinsch, S.; Kinkel, L.; Smanski, M.; Harcombe, W.R.; Breitling, R.; Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
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Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Rights holder
University of Manchester
jack.connolly@manchester.ac.uk
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