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Barraclough, T.G.; Sant, D.G.; Wong, E.L.Y.

Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford

https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30
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This dataset is available under the terms of the Open Government Licence

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The data comprises both compositional data and growth assay data from an experiment of wild bacterial communities in Wytham Woods, Oxford. The communities live in puddles of water in tree-holes formed by the roots of beech trees. We manipulated the pH of tree-holes during the experiment for comparison with control tree-holes that were unmanipulated. Compositional data comprises the frequency of different Amplicon Sequencing Variants and derived measures of species diversity for samples taken over time (up to 12 weeks from the start of the experiment). The growth assay data comprises measurements of growth rates of community samples and isolates back in the laboratory, when grown on beech tea media manipulated to have different pHs.
Publication date: 2025-10-20
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More information

View numbers valid from 20 October 2025 Download numbers valid from 20 October 2025 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid

Temporal information

Temporal extent
2021-05-11    to    2021-09-29

Provenance & quality

A field experiment was conducted in summer 2021 that surveyed bacterial communities in tree-hole puddles formed by beech tree roots at Wytham Woods, Oxford. The experiment added lime (alkali) to a set of tree-holes and compared changes in bacterial communities to control tree-holes without liming. Samples were collected before and after the manipulation, and some of these were used for growth assays in the laboratory: growing different combinations of isolates and communities on beech tea medium with modified pH.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Barraclough, T.G.; Sant, D.G.; Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30

Correspondence/contact details

Timothy Giles Barraclough
University of Oxford
 tim.barraclough@biology.ox.ac.uk

Authors

Barraclough, T.G.
University of Oxford
Sant, D.G.
Rutgers, The State University of New Jersey
Wong, E.L.Y.
Senckenberg Biodiversity and Climate Research Centre

Other contacts

Rights holder
University of Oxford
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
INSPIRE theme
Environmental Monitoring Facilities
Keywords
bacterium , Biodiversity , deciduous tree , ecological community , evolution , Evolutionary ecology , field experiment , field study , microbiology , pH
Funding
Natural Environment Research Council Award: NE/V011596/1