Lugli, L.F. et al

Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018

The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths.

Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).

The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship).

Publication date: 2020-04-02

Get the data

This dataset is available under the terms of the Open Government Licence

Format of the data: Comma-separated values (CSV)

You must cite: Lugli, L.F.; Quesada, C.A.N.; Andersen, K.M.; Mercado, L. M.; Di Ponzio, R.; Ribeiro, G.; Assis, R. L. ; Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre.


© Natural Environment Research Council


Study area
Temporal extent
2018-01-01    to    2018-09-30

Provenance & quality

Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.


This dataset is included in the following collections

Amazon Fertilization Experiment (AFEX) in Central Amazon, Brazil (2017 - 2020)

Correspondence/contact details

Laynara Lugli
National Institute of Amazonian Research, Brazil
Prof. Iain Hartely
University of Exeter


Lugli, L.F.
National Institute of Amazonian Research, Brazil
Quesada, C.A.N.
National Institute of Amazonian Research, Brazil
Andersen, K.M.
Nanyang Technological University, Singapore
Mercado, L. M.
University of Exeter
Di Ponzio, R.
National Institute of Amazonian Research, Brazil
Ribeiro, G.
National Institute of Amazonian Research, Brazil
Assis, R. L.
Natural History Museum, University of Oslo, Norway
Hartley, I.P.
University of Exeter

Other contacts

Environmental Information Data Centre
NERC Environmental Information Data Centre

Additional metadata

Topic categories
Biota , Environment
Environmental survey Soil
Natural Environment Research Council
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Last updated
01 March 2021 12:32