This dataset is under embargo and will be made available by 4 January 2027 at the latest Find out more »
Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids
https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15
Cite this dataset as:
Parker, M.E.; Zytynska, S.E. (2025). Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15
Download/Access
This dataset is under embargo and will be made available by 4 January 2027 at the latest Find out more »
This dataset comprises whole-leaf and headspace metabolomics of barley plants (Hordeum vulgare) that were root-inoculated with three rhizobacterial treatments: Acidovorax radicis N35, Bacillus subtilis B171, or no bacteria (control). Plants were also either infested with Sitobion avenae aphids or left uninfested as controls. Plants were grown under controlled conditions in glasshouse (leaf extracts) or climate chamber (headspace analysis).
The purpose of these experiments was to compare metabolomic profiles across rhizobacterial treatments, and when aphids were feeding on plants, as an assessment of wider-community effects on rhizosphere inoculation of barley plants.
The purpose of these experiments was to compare metabolomic profiles across rhizobacterial treatments, and when aphids were feeding on plants, as an assessment of wider-community effects on rhizosphere inoculation of barley plants.
Publication date: 2025-11-13
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Format
Comma-separated values (CSV)
Provenance & quality
Barley plants (Hordeum vulgare, cultivars Irina (KWS seeds) and Firefoxx (Elsoms seeds)), were treated with Acidovorax radicis N35, Bacillus subtilis B171, or a control, and either infested with Sitobion avenae aphids or left uninfested.
In the first experiment, whole leaves were sampled, metabolites extracted with hexane, derivatised and analysed using an Agilent 7250 GC-qToF-MS equipped with an HP-5MS column (30 m × 0.25 mm × 0.25 μm).
In the second experiment, plants were enclosed in transparent PET film bags (31 cm × 30 cm; Toppits® Bratschlauch, Melitta Group, Germany) sealed at both ends and headspace volatiles were collected using Tenax probes and analysed on a GC-qToF-MS coupled with thermal desorption. Data were processed using MS-DIAL, with blank subtraction, internal standard normalisation, QC correction, and log transformation. Annotated peaks were matched against spectral libraries.
Features were assigned metabolite numbers to enable unbiased comparisons, including unknowns, across both experiments.
In the first experiment, whole leaves were sampled, metabolites extracted with hexane, derivatised and analysed using an Agilent 7250 GC-qToF-MS equipped with an HP-5MS column (30 m × 0.25 mm × 0.25 μm).
In the second experiment, plants were enclosed in transparent PET film bags (31 cm × 30 cm; Toppits® Bratschlauch, Melitta Group, Germany) sealed at both ends and headspace volatiles were collected using Tenax probes and analysed on a GC-qToF-MS coupled with thermal desorption. Data were processed using MS-DIAL, with blank subtraction, internal standard normalisation, QC correction, and log transformation. Annotated peaks were matched against spectral libraries.
Features were assigned metabolite numbers to enable unbiased comparisons, including unknowns, across both experiments.
Licensing and constraints
This dataset is under embargo and will be made available by 4 January 2027 at the latest Find out more »
This dataset will be available under the terms of the Open Government Licence 
Cite this dataset as:
Parker, M.E.; Zytynska, S.E. (2025). Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15
Correspondence/contact details
Authors
https://orcid.org/0009-0007-4602-8382 Other contacts
Rights holder
University of Liverpool
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk