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Howard, S.; McCarthy, R.R.

Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions

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This dataset is under embargo and will be made available by 4 June 2025 at the latest   Find out more »

https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control.
The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics.
Publication date: 2024-10-28
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View numbers valid from 28 October 2024 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Temporal information

Temporal resolution
24 hours

Provenance & quality

Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted.

Licensing and constraints

This dataset is under embargo and will be made available by 4 June 2025 at the latest   Find out more »

This dataset is licensed under a Creative Commons Attribution 4.0 International Licence

Cite this dataset as:
Howard, S.; McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015

Correspondence/contact details

McCarthy, R.R.
Brunel University London
Kingston Lane
Uxbridge
UB8 3PH
UNITED KINGDOM
 Ronan.mccarthy@brunel.ac.uk

Authors

Howard, S.
Brunel University London
McCarthy, R.R.
Brunel University London

Other contacts

Rights holder
Brunel University London
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
health
INSPIRE theme
Human Health and Safety
Keywords
Biofilm , Escherichia coli , plastic degradation , Pollution , polyesterase
Funding
Natural Environment Research Council Award: NE/X010902/1
Last updated
29 October 2024 17:19