Howard, S.; McCarthy, R.R.
Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions
This dataset is under embargo and will be made available by 4 June 2025 at the latest Find out more »
Cite this dataset as:
Howard, S.; McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
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This dataset is under embargo and will be made available by 4 June 2025 at the latest Find out more »
https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control.
The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics.
The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics.
Publication date: 2024-10-28
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Format
Comma-separated values (CSV)
Temporal information
Temporal resolution
24 hours
Provenance & quality
Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted.
Licensing and constraints
This dataset is under embargo and will be made available by 4 June 2025 at the latest Find out more »
This dataset is licensed under a Creative Commons Attribution 4.0 International Licence
Cite this dataset as:
Howard, S.; McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Correspondence/contact details
McCarthy, R.R.
Brunel University London
Kingston Lane
Uxbridge
UB8 3PH
UNITED KINGDOM
Ronan.mccarthy@brunel.ac.uk
Uxbridge
UB8 3PH
UNITED KINGDOM
Authors
Howard, S.
Brunel University London
McCarthy, R.R.
Brunel University London
Other contacts
Rights holder
Brunel University London
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Additional metadata
Funding
Natural Environment Research Council Award: NE/X010902/1
Last updated
29 October 2024 17:19