Hodgson, C.J.

Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform

The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning.

Publication date: 2020-05-18

Get the data

Format of the data: Comma-separated values (CSV)

You must cite: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce

 

© Natural Environment Research Council

Where/When

Study area
Temporal extent
2016-11-01    to    2016-11-30

Provenance & quality

Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong.

The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.

Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.

Correspondence/contact details

Dr. Chris Hodgson
Rothamsted Research North Wyke
 chris.hodgson@rothamsted.ac.uk

Author

Hodgson, C.J.
Rothamsted Research North Wyke

Other contacts

Custodian
Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
 info@eidc.ac.uk
Rights Holder
Rothamsted Research
 comms@rothamsted.ac.uk

Additional metadata

Topic categories
Biota , Environment
Keywords
Antibiotic resistant gene abundance,  Environmental survey North Wyke Farm Platform
INSPIRE Theme
Environmental Monitoring Facilities
Funding
Natural Environment Research Council
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Last updated
19 May 2020 16:14