Hodgson, C.J.
Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform
Cite this dataset as:
Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
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This dataset is available under the terms of the Open Government Licence
https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning.
Publication date: 2020-05-18
View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)
Format
Comma-separated values (CSV)
Spatial information
Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Temporal information
Temporal extent
2016-11-01 to 2016-11-30
Provenance & quality
Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong.
The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.
Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.
Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
© Natural Environment Research Council
Correspondence/contact details
Author
Other contacts
Rights holder
Rothamsted Research
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
info@eidc.ac.uk
Additional metadata
Keywords
Antibiotic resistant gene abundance , North Wyke Farm Platform
Funding
Natural Environment Research Council Award: NE/N019792/1
Last updated
08 February 2024 17:32