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Griffiths, R.; Edwards, F.; Scarlett, P.; Webb, G.; Sarlej, R.; Thomson, B.

Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments

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© UK Centre for Ecology & Hydrology

This dataset is made available under the terms of the Open Government Licence

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https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367
This dataset contains operational taxonomic units for epilithon eukaryotes (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wolf River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
Publication date: 2014-05-06
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More information

View numbers valid from 01 June 2023 Download numbers valid from 20 June 2024 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid

Temporal information

Temporal extent
2013-10-01    to    2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wolf River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Licensing and constraints

This dataset is made available under the terms of the Open Government Licence

Cite this dataset as:
Griffiths, R.; Edwards, F.; Scarlett, P.; Webb, G.; Sarlej, R.; Thomson, B. (2014). Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367

© UK Centre for Ecology & Hydrology

Related

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

Edwards, F.
UK Centre for Ecology & Hydrology
Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM
 enquiries@ceh.ac.uk

Authors

Griffiths, R.
Centre for Ecology & Hydrology
Edwards, F.
Centre for Ecology & Hydrology
Scarlett, P.
Centre for Ecology & Hydrology
Webb, G.
Centre for Ecology & Hydrology
Sarlej, R.
Centre for Ecology & Hydrology
Thomson, B.
Centre for Ecology & Hydrology

Other contacts

Rights holder
UK Centre for Ecology & Hydrology
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
environment
inlandWaters
location
INSPIRE theme
Land Use
Keywords
Biodiversity , Environmental monitoring facilities , genetics , hydrography , microbiology , microbiology , soil , South West project , Species distribution , Tellus
Last updated
21 March 2025 09:22