Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018

https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

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The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths.

Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).

The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship).

Publication date: 2020-04-02

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Format

Comma-separated values (CSV)

Spatial information

Study area

Spatial representation type

Tabular (text)

Spatial reference system

WGS 84

Temporal information

Temporal extent

2018-01-01 to 2018-09-30

Provenance & quality

Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:

Lugli, L.F.; Quesada, C.A.N.; Andersen, K.M.; Mercado, L.M.; Di Ponzio, R.; Ribeiro, G.; Assis, R.L.; Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre. https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

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